FIG. 3.
Loss of LRH-1 in hepatocytes leads to cholic acid deficiency and alters BA pool size, composition, and excretion. Total BA pool (A, C, and E) and BA composition (B, D, and F) in livers/gallbladders, intestinal extracts (A and B), plasma (C and D), and feces (E and F) of LRH-1L2/L2 (n = 4) and LRH-1hep−/− (n = 14) mice. Unconjugated CA and TCA, MCA and TMCA, UDCA and TUDCA, and CDCA/DCA and TCDCA/TDCA were measured in the enterohepatic compartment and in plasma. LCA and other unconjugated BA species were measured for feces samples. Statistical differences between both genotypes were calculated from the sum of liver/gallbladder and intestinal extracts. *, P < 0.05; **, P < 0.01; ***, P < 0.001.