Table 1.
Mutant and morphants examined in the present study. Larval stages and number of specimens analysed are detailed in the table, together with a brief description of the overall phenotype and the morphology of the intraocular vasculature
| NAME | CATEGORY | AGE | n | GENERAL PHENOTYPE | HYALOID -RETINAL PHENOTYPE |
| MAGP1 | MORPHANT Microfibril associated glycoprotein 1 |
3 dpf | 15 | Dilated brain and caudal vessels. Irregular lumen of axial vasculature (Chen et al. 2006) |
Stagnated growth of hyaloid vasculature with less and thicker branches. Aggregation of vascular endothelial cells at the posterior lens |
| 5 dpf | 15 | ||||
| HS6ST2 | MORPHANT heparan sulfate sulfotransferase 2 |
5 dpf | 15 | Over-lumenized vessels and defective branching in caudal vein plexus (Chen et al. 2005) |
Hyaloid vasculature with scarce and oversized branches that display an aberrant patterning. |
| Syn 2 | MORPHANT Morphant of syndecan 2 |
5 dpf | 6 | Aberrant or absent sprouting in the intersegmental vessels (Chen et al. 2004) |
Sparse disorganized endothelial cells surrounding very small lens. |
| Sppl2b | MORPHANT Signal peptide peptidase like protein 2b |
5 dpf | 10 | Erythrocyte accumulation in an enlarged caudal vein (Krawitz et al. 2005) |
NO PHENOTYPE |
| Mab21l2 | MORPHANT Male abnormal 21 like-2 protein |
5 dpf | 5 | Microphthalmia. Apoptosis in the developing retina and lens (Kennedy et al. 2004) |
Thicker vessels that cover only posterior part of the lens. |
| obd | VASCULAR MUTANT Plexin-D1 out of bounds |
3 dpf | 5 | Mispatterning of the trunk intersegmental vessels (variable severity). ~10% of mutants die before 6 dpf (Childs et al. 2002; Torres-Vazquez et al. 2004) |
Mis-patterning of the hyaloid vessels (variable severity). Abnormal branching and extra interconnections obvious at 5 dpf. Atypical loops, tortuosity and increased number of vessels in the retinal vasculature of the adult. |
| 5 dpf | 20 | ||||
| 6 dpf | 5 | ||||
| 10 dpf | 5 | ||||
| 20 dpf | 3 | ||||
| Adult | 10 | ||||
| ace | VASCULAR MUTANT FGF8 Hypomorph acerebellar |
5 dpf | 5 | General CNS abnormalities. Absence of vessels in the dorsal brain. Abnormal heart development (Reifers et al. 1998; Reifers et al. 2000) |
NO PHENOTYPE |
| arl | LENS MUTANT Laminin alpha1 arrested lens |
3 dpf | 3 | Abnormal eye development. Lens development halted at 24 hpf. Lack of any lens structure from 48 hpf. (Vihtelic et al. 2001; Vihtelic and Hyde 2002; Semina et al. 2006) |
Complete absence of hyaloid vessels in all stages analysed |
| 4 dpf | 3 | ||||
| 5 dpf | 3 | ||||
| 6 dpf | 3 | ||||
| mgf | LENS MUTANT/Unknown margin affected |
4 dpf | 7 | Abnormal eye development from 3 dpf. Very small lens (Unpublished) |
At 4 dpf rudimentary hyaloid vasculature (fewer vessels and branches) on lens. At 5 dpf complete absence of hyaloid vasculature on small lens |
| 5 dpf | 8 | ||||
| dsl | LENS MUTANT/Unknown disrupted lens |
5 dpf | 5 | Abnormal eye and lens development. (Vihtelic et al. 2001; Vihtelic and Hyde 2002) |
NO PHENOTYPE |
| 6 dpf | 4 | ||||
| 8 dpf | 5 | ||||
| fe | LENS-VASCULAR MUTANT/Unknown fused eyes |
3 dpf | 7 | Synophthalmia. Abnormal/absent intersegmental blood vessels. (Unpublished) |
No hyaloid vessels on the lens |
| plt | LENS MUTANT/Unknown platinum |
4 dpf | 3 | Defects in melanin pigmentation. Photoreceptor and RPE degeneration from 5 dpf. (Vihtelic et al. 2001; Vihtelic and Hyde 2002) |
Premature detachment of hyaloid vessels from the lens at 4 dpf. Less vessels and less patterning of the branches covering only posterior lens at 6 dpf. |
| 5 dpf | 3 | ||||
| 6 dpf | 3 | ||||
| lop | LENS MUTANT/Unknown lens opaque |
6 dpf | 4 | Lens opacity (Vihtelic et al. 2005) |
NO PHENOTYPE |