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. Author manuscript; available in PMC: 2008 Jul 15.
Published in final edited form as: Exp Cell Res. 2007 May 18;313(12):2597–2610. doi: 10.1016/j.yexcr.2007.05.009

Figure 1.

Figure 1

Western blot analysis of MDCK II cell monolayers incubated with 10 mM MBCD for 0, 0.5 or 2 h. Monolayers were lysed either in TX-100 or CHAPS at 4°C and centrifuged at 1000 g for 30 min. Low speed pellets (LSP) and aliquots of the low speed supernatants (LSS) were retrieved. The remaining low speed supernatants were centrifuged at 4°C for 18 h at 107,600 g. High-speed supernatants (HSS) and high-speed pellets (HSP) were harvested. All supernatant and pellet fractions were processed for Western blotting and probed for occludin, claudin-1, -2, -3, -4, -7, caveolin-1, annexin-2, ZO-1 and actin. The data shown are representative of those obtained from a minimum of two independent experiments using TX-100 and CHAPS.