Figure 11. Effect of AP5 on interneuron responses measured by 2-photon Ca²⁺ imaging.

(A) Odor-evoked Ca²⁺ signals in interneurons before, during and after application of AP5 (stimulus: food odor). (B) Average somatic Ca²⁺ signals before (control) and during application of AP5, normalized to control. Error bars show standard deviation. (C) Cumulative distribution of Ca²⁺ signal amplitudes before (black) and during (red) application of AP5. (D) Comparison of Ca²⁺ signal amplitudes evoked by the same odors in the same interneurons before and during application of AP5. r, Pearson correlation coefficient. Inset shows the density of data points in the boxed region. Lines are diagonals with slope one. (E) Left: interneuron odor responses ranked according to the Ca²⁺ signal before application of AP5. Data were pooled over all cells, odors and anminals (n = 14884 responses). Right: Responses of the same interneurons to the same odors in the presence of AP5, ranked in the same order as in the control. Inset shows an enlargement of a subregion to demonstrate that low-amplitude values are interspersed between high amplitude values. The visual impression in the full diagram that many amplitudes are increased during AP5 treatment is therefore an artifact caused by crowding of bars in the graph.