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. 1999 Nov 29;147(5):995–1008. doi: 10.1083/jcb.147.5.995

Figure 4.

Figure 4

Rescue of abnormal localization of SERCA and ryanodine receptors in ankyrin-B (−/−) cardiomyocytes by transfection with cDNA encoding 220-kD ankyrin-B–GFP. A, B, E, and F show ankyrin-B (+/+) cardiomyocytes (A and E), and ankyrin-B (−/−) cardiomyocytes (B and F), triple-labeled for F-actin with biotin phalloidin/Cy5-labeled avidin (left panels), ankyrin-B (A, B, E, and F, right panels) and either SERCA (A and B, middle panels) or ryanodine receptor (E and F, middle panels). C, D, G, and H show ankyrin-B (−/−) cardiomyocytes transfected with cDNA encoding either 220-kD ankyrin-B–GFP (C and G), or 190-kD ankyrin-G–GFP (D and H), triple-labeled for F-actin with phalloidin (C, D, G, and H, left panels), GFP (C, D, G, and H, right panels) and either SERCA (C and D, middle panels) or ryanodine receptor (G and H, middle panels). Culture of cardiomyocytes, transfections, and immunofluorescence microscopy were performed as described in Materials and Methods. Note recovery of normal SERCA and ryanodine receptor distribution after transfection with ankyrin-B–GFP but not ankyrin-G–GFP. Bars, 20 μm.