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Ankyrin-B polypeptides in skeletal muscle are associated with a dense membrane vesicle fraction. (A) Coomassie blue–stained SDS polyacrylamide gel of starting material (T) and sucrose gradient fractions of rat skeletal muscle microsomes sedimented to equilibrium (16 h at 150,000 g) on a sucrose density gradient (see Materials and Methods). (B) Immunoblots of sucrose gradient fractions visualized in A using antibodies against ankyrin-B, ryanodine receptor types 1 and 2, and SERCA1.
