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. 1999 Nov 29;147(5):945–958. doi: 10.1083/jcb.147.5.945

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© 1999 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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Disruption of cytoskeleton induces changes in the localization of wt vRNA and vRNA-ΔM. Protoplasts treated at midstages of infection with 10 μM oryzalin for 2 h induces the accumulation of vRNA in enlarged fluorescent bodies surrounding the nucleus (vRNA/Oryzalin). Treatment at early stages of infection with cytochalasin D delays the formation of large bodies, and the pattern of vRNA accumulation was similar to that observed in infection by vRNA-ΔM (vRNA/Cytoch D). In protoplasts infected with vRNA-ΔM and treated at early stages of infection with cytochalasin D, vRNA-ΔM accumulated in filaments and narrow structures at the periphery of the cell (vRNA-ΔM/Cytoch D). Bars, 10 μm.

Disruption of cytoskeleton induces changes in the localization of wt vRNA and vRNA-ΔM. Protoplasts treated at midstages of infection with 10 μM oryzalin for 2 h induces the accumulation of vRNA in enlarged fluorescent bodies surrounding the nucleus (vRNA/Oryzalin). Treatment at early stages of infection with cytochalasin D delays the formation of large bodies, and the pattern of vRNA accumulation was similar to that observed in infection by vRNA-ΔM (vRNA/Cytoch D). In protoplasts infected with vRNA-ΔM and treated at early stages of infection with cytochalasin D, vRNA-ΔM accumulated in filaments and narrow structures at the periphery of the cell (vRNA-ΔM/Cytoch D). Bars, 10 μm.