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. 1999 Nov 29;147(5):945–958. doi: 10.1083/jcb.147.5.945

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© 1999 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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Intracellular distribution of TMV minus-strand RNA in protoplasts infected with wt RNA. Protoplasts collected at early stages of infection (4–6 hpi) were fixed and hybridized with a fluorescein-RNA probe that recognized the complementary strand of vRNA. (A) Minus-strand RNA accumulation in a perinuclear pattern and in small cytoplasmic patches. (B) RNA surrounding the nucleus with a discrete region that lacked hybridization. (C and D) Nuclei of protoplasts at 6 hpi showing vRNA-free zone. A, C, and D correspond to single optical sections, while B corresponds to a projection of serial optical sections. N, nucleus. Bars: (A and B) 10 μm; (C and D) 5 μm.