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. 1999 Nov 29;147(5):945–958. doi: 10.1083/jcb.147.5.945

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© 1999 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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vRNA colocalizes with the replicase. Protoplasts infected with wt vRNA were fixed at early stages of infection and processed for immunofluorescence using antireplicase antibody and in situ hybridization for detecting vRNA. Most of the replicase (red signal) was localized in sites were vRNA (green signal) accumulated. Merging both images demonstrates colocalization of both signals (yellow in merged image). N, nucleus. Bars, 10 μm.