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. 1999 Nov 29;147(5):945–958. doi: 10.1083/jcb.147.5.945

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© 1999 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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vRNA colocalizes with BiP (ER marker). Protoplasts infected with wt vRNA were fixed at early stages of infection and processed for immunofluorescence using anti–BiP antibody and in situ hybridization. Most of the BiP (red) was associated with sites that contain vRNA (green). Merging the two images demonstrates colocalization of the signals (yellow in merged image). Bars, 10 μm.