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. 1999 Nov 29;147(5):945–958. doi: 10.1083/jcb.147.5.945

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© 1999 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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vRNA colocalizes with microtubules. Protoplasts infected with wt vRNA were fixed and processed for immunofluorescence using antitubulin antibody and in situ hybridization. (A) Most of the tubulin (red) was associated with filaments that coaligned with the filaments that contain vRNA (green). Merging the images demonstrates colocalization of the signals (yellow in merged image). (B) Some of the bright fluorescent patches containing vRNA (green) appear to be aligned with microtubule filaments (red). Bars, (A) 10 μm; (B) 2 μm.