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. 1999 Nov 29;147(5):1049–1062. doi: 10.1083/jcb.147.5.1049

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© 1999 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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Embryonic motoneurons express Fas and FasL polypeptides in vivo. Western blot analysis was performed using specific antibodies to FasL and Fas. (A) Antibodies to FasL specifically label a 38-kD band in P17 mouse thymus, but not in heart. In ventral spinal cord from rodent embryos dissected at the stage at which motoneuron PCD is about to begin, FasL is abundant. It is also clearly present in freshly purified motoneurons from E14 rat. (B) Controls for the specificity of immunoblot-purified anti-Fas antibodies (M-20) using thymus extracts from P17 mice of the C57BL/6 strain. Wild-type (+/+) and Fas mutant (lpr/lpr) samples are shown. (C) Using immunoblot-purified antibodies, Fas is abundant in adult rat heart but not in eye. Both ventral spinal cord and freshly purified motoneurons from embryos show clear expression of a single band corresponding to Fas.