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. 1999 Nov 29;147(5):1049–1062. doi: 10.1083/jcb.147.5.1049

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© 1999 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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Motoneurons in culture coexpress Fas and FasL. Immunolabeling of purified E12.5 mouse motoneurons cultured for 1 d in the presence of neurotrophic factors. (A) Cultured motoneurons labeled using blot-purified rabbit anti-Fas (M-20). Note punctate labeling of cell membranes and more diffuse labeling of nucleus. There was variation in intensity from cell to cell (not apparent in this field). (B) Control in which the same primary antibody was preincubated with the immunogenic peptide. Preincubation with an irrelevant peptide did not reduce staining (data not shown). (C) Immunostaining using a rat mAb against FasL (H11). Note the strong homogeneous staining. (D) Double immunolabeling for Fas (green) and FasL (red), showing the death receptor and its ligand present at the cell surface of the same motoneuron. Controls using an irrelevant rat mAb of the same class or purified rabbit antibodies gave no significant staining (data not shown).