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Histochemical determination of mineral in control (RCAS) and MGP-overexpressing (RCAS-MGP) cultures. Cultures similar to those described in Fig. 7 legend were grown for ∼2 wk until confluent. Cultures (in duplicate) were grown for an additional 7 d in medium A in the absence (none) or presence of 10 μM vitamin K1 or K2. At the end of the treatment, cultures were stained with alizarin red to reveal mineralization.
