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. 1999 Nov 29;147(5):929–936. doi: 10.1083/jcb.147.5.929

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© 1999 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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Localized BK application. a, Simulations. [Ca²⁺]_cyt is shown for simulations of focal 500 nM BK application to the soma, neurite, and growth cone. b, Experiments. Cells were loaded with fura-2, 340/380 ratio pairs collected with a cooled CCD camera, and results displayed in pseudocolored [Ca²⁺]. 500-nM BK was focally applied (arrowheads) by pressure ejection from a micropipette in a flow chamber at time = 0 s. Bars, 25 μm. Note that the pseudocolor [Ca²⁺] scales are not identical.

Localized BK application. a, Simulations. [Ca²⁺]_cyt is shown for simulations of focal 500 nM BK application to the soma, neurite, and growth cone. b, Experiments. Cells were loaded with fura-2, 340/380 ratio pairs collected with a cooled CCD camera, and results displayed in pseudocolored [Ca²⁺]. 500-nM BK was focally applied (arrowheads) by pressure ejection from a micropipette in a flow chamber at time = 0 s. Bars, 25 μm. Note that the pseudocolor [Ca²⁺] scales are not identical.