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. 1999 Nov 29;147(5):1109–1122. doi: 10.1083/jcb.147.5.1109

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© 1999 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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ECM expression in long bones. (A and B) Safranin orange (SO) and van Kossa (vK) double staining show reduced proteoglycan content in mutant (B) as compared with wild-type (A) cartilage and the absence of mineralization of longitudinal septa in the lower hypertrophic zone (1 h) of the mutant growth plate (A and B). Also, note the transversally oriented trabecular bones in the mutant (arrows). (C–H) Immunostaining of perlecan and collagen types II (Col2) and X (Col10) on consecutive sections of elbows from normal and perlecan-null E15.5 embryos. Perlecan is present in normal cartilage, in the periosteum/perichondrium, and in the surrounding connective tissues (C). In mutant embryos, perlecan staining is absent (D). The distribution of collagen types II (E and F) and X (G and H) is similar in normal (E and G) and perlecan-null (F and H) cartilage. Bar: (A–D and G and H) 100 μm; 50 μm in E and F.