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. 2000 Feb 21;148(4):769–778. doi: 10.1083/jcb.148.4.769

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© 2000 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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An apical chimera and the Na,K-ATPase β subunit colocalize at the apical membrane. Cells expressing chimera IV were fixed and stained for the presence of either the chimera or the Na,K-ATPase β subunit. The cells expressing the apical chimera (A, en face and C, xz section) express the Na,K-ATPase β subunit at the apical surface, where it is presumably assembled with the chimera, as well as at the basolateral surface, where it is normally found in association with the endogenous Na,K-ATPase α subunit (B, en face and D, xz section).