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. 2000 Feb 21;148(4):811–824. doi: 10.1083/jcb.148.4.811

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© 2000 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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Constitutive shedding of syndecan-1 and -4 is inhibited by peptide hydroxamates but not by TIMP-3. SVEC4-10 cells were incubated for 14 h in the presence or absence of the indicated concentration of (A and B) peptide hydroxamate BB-2116 and BB-1101, or (C and D) TIMP-3. The syndecan-1 (A and C) and syndecan-4 (B and D) ectodomains in the conditioned media were assayed by dot blot analysis as in Fig. 1. Quantitation was done as in Fig. 1 and each point represents the mean ± SD (n = 3). TIMP-1 and TIMP-2 (20 μM) did not inhibit constitutive shedding in this assay, and treatment with the PTK, PKC, or MAPK inhibitors alone did not affect the level of shedding compared with untreated controls during the 2 h shedding assays.