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. 2000 Feb 21;148(4):811–824. doi: 10.1083/jcb.148.4.811

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© 2000 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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Receptor- and stress-activated shedding is inhibited by TIMP-3. (A and B) SVEC4-10 cells were treated with or without 10 ng/ml EGF or 100 μM TRAP, or (C and D) NMuMG cells were treated with or without 100 μM ceramide for 2 h in the presence or absence of 20 μg/ml TIMP-3. The syndecan-1 (A and C) and syndecan-4 (B and D) ectodomains in the conditioned media were assayed by dot blot analysis as in Fig. 1. Quantitation was done as in Fig. 1 and each point represents the mean ± SD (n = 3). For each shedding agonist assayed, treatment with TIMP-1, -2, or -3 alone (20 μM) had no effect on the level of shedding compared with the untreated control.