FIG. 3.

Determination of protein interaction domains on ORF1p. (A) Two-hybrid assays were performed to determine binding domains for DdTBP and DdBrf1 on ORF1p. The selection plates contained minimal medium without histidine that was supplemented with 5 mM 3-AT. Combinations of two-hybrid vectors are listed; the left column represents proteins expressed from pBT vectors, and the right column lists proteins cloned into the pTRG vector. (B) Presentation of ORF1p deletions tested for interaction with ORF1p (dimerization), DdTBP, and DdBrf1. + and − refer to growth of transformants on selection plates containing 5 mM 3-AT.