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. 2007 Oct 8;27(24):8698–8712. doi: 10.1128/MCB.00948-07

FIG. 10.

FIG. 10.

Nephrin and Neph1 cooperate to induce actin polymerization in a Nck- and Grb2-dependent fashion. Quantification of actin tail-forming efficiency. As described in the text, combinations of CD16/CD7/NephrinCD, full-length wild-type Neph1, CD16/CD7/NephrinCD(TM), or full-length wild-type Neph1(DM) were expressed with Nck and/or Grb2 in NIH 3T3 cells as indicated. The number of actin tails per individual cell was counted in 30 cells for each condition. Each experiment was repeated four times; the graph represents the mean number of tails per cell ± standard deviation. The table provides results of the single-tailed paired t test used to compare groups. Neph1(DM), Y-to-F mutation at Y637 and Y638; Nephrin(TM), Y-to-F mutation at Y1191, Y1208, and Y1232. NA, not applicable.