TABLE 1.
Statistical analyses of FRAP recovery rates of single- and double-residue mutations on NS and GNL3L
| Protein or mutation(s) (mutant name) | % FRAP recovery (mean ± SD) at:a
|
||
|---|---|---|---|
| 5 s | 10 s | 40 s | |
| NS | 74.0 ± 3.3 | 84.7 ± 2.4 | 94.6 ± 2.9 |
| P157F | 68.4 ± 4.8 ** | 76.9 ± 4.4 ** | 87.6 ± 4.0 ** |
| S178I | 79.0 ± 3.0 ** | 86.7 ± 2.5 ** | 93.4 ± 3.8 |
| K232A | 82.2 ± 2.4 ** | 89.3 ± 2.0 ** | 94.8 ± 2.5 |
| F257L | 72.9 ± 6.0 | 79.7 ± 5.6 ** | 86.8 ± 5.4 ** |
| P157F-S178I (D1) | 77.0 ± 4.4 * | 84.6 ± 3.5 | 91.4 ± 4.2 ** |
| K232A-F257L (D2) | 73.8 ± 4.6 | 83.1 ± 4.0 | 91.1 ± 4.1 ** |
| GNL3L | 90.2 ± 3.2 | 94.4 ± 2.6 | 96.7 ± 3.2 |
| F146P | 90.5 ± 1.9 | 94.6 ± 2.2 | 97.6 ± 3.0 |
| I168S | 89.9 ± 3.8 | 93.6 ± 2.9 | 97.4 ± 2.6 |
| A227K | 85.5 ± 4.0 ** | 90.4 ± 2.8 ** | 96.5 ± 3.0 |
| L254F | 89.6 ± 4.1 | 93.0 ± 4.0 | 97.7 ± 4.5 |
| F146P-I168S (K1) | 90.0 ± 3.2 | 93.4 ± 2.8 | 96.9 ± 2.9 |
| A227K-L254F (K2) | 77.4 ± 8.5 ** | 84.3 ± 7.6 ** | 95.3 ± 9.3 |
a
*, P value is <0.01 compared to NS or GNL3L; **, P value is <0.001 compared to NS or GNL3L.