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. 2007 Oct 15;27(24):8637–8647. doi: 10.1128/MCB.00393-07

FIG. 6.

FIG. 6.

Functional HDAC6 and Hsp90 are required for efficient Rac1 activation. (A) Wild-type MEFs were stimulated with 50 ng/ml PDGF-BB for 8 min. Colocalization of Rac1 (b) with F-actin (a) was observed at dorsal ruffle (filled arrowheads) and F-actin dots (open arrowheads) using anti-Rac1 antibody and phalloidin-Alexa Fluor 488. Bar = 10 μm. (B) Rac1 activation in wild-type (WT) and HDAC6 KO MEFs was evaluated by GST-PAK1-CRIB pull-down assay. A diminished Rac1 activation in response to PDGF-BB treatment was observed in HDAC6 KO MEFs. (C) Pretreatment of wild-type MEFs with vehicle control, wortmannin (Wort) (100 nM for 30 min as another control), or geldanamycin at indicated concentrations for 2 h led to reduced Rac1 activation by PDGF-BB treatment (50 ng/ml for 10 min) in a GST-PAK1-CRIB pull-down assay. Note that wortmannin treatment inhibits PI3K function and Rac1 activation (23, 44). DMSO, dimethyl sulfoxide.