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. 2007 Oct 15;27(24):8834–8847. doi: 10.1128/MCB.00974-07

FIG. 1.

FIG. 1.

Expression of mAPE1 in response to sodium arsenite in 10T½ cells. (A) Dose-response experiments. Confluent cells were treated for 30 min in medium containing the indicated concentration of arsenite and then washed and incubated in fresh medium for 2 h before harvesting for Northern blotting analysis. (B) Kinetics. Confluent cells were treated with 50 μM arsenite for the indicated times, then washed, and incubated in fresh medium for 2 h before harvesting for Northern blotting analysis. (C and D) Cellular viability under conditions corresponding to the experiments shown in panels A and B, respectively. (E) Four hours after arsenite treatment, Ape1 protein levels were detected by immunoblotting. (F) Six hours after arsenite treatment, the AP endonuclease assay of Ape1 activity was performed. The 35-mer band is the substrate, and the 12-mer band is the AP endonuclease cleavage product. AP, purified hApe1 as a positive control. Three independent sets of assays were performed and normalized to GAPDH mRNA for Northern blotting or to β-actin for immunoblotting. Standard deviations are indicated by error bars. Values that were significantly different (P < 0.05) from the value for the untreated control are indicated by an asterisk.