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. 2007 Oct 8;27(24):8571–8582. doi: 10.1128/MCB.01350-07

FIG. 6.

FIG. 6.

Expression of epicardial markers in Sp3−/− hearts. Sections of E12.5 (a to f) and E14.5 (g to l) hearts stained for Wt1 and E-cadherin. AVC region of an E12.5 WT heart (a) with detail of the left ventricle (LV) (b) showing the epicardial (EP) covering and the EPDCs in the subepicardial layer (arrows). The latter are missing in the mutant (e) taken from a similar position in a heart (d) displaying a common atrioventricular orifice, a thin spongy myocardium, and a deficient ventricular septum (VS). At E14.5 Wt1-stained EPDCs (arrows in panel g) are missing around the developing coronary vascular system (j). In the compact layer of the myocardium, fewer Wt1-stained EPDCs (arrow) are found in the mutant (k) than in the WT heart (h). In the Sp3−/− hearts E-cadherin (f and l) is upregulated compared to that in WT hearts (c and i), where EPDCs (arrows) are also located in the subepicardial region. Ao, aorta; LA, left atrium; RV, right ventricle. Bars, 60 μm (a, d, g, and j) and 30 μm (b, c, e, f, h, i, k, and l).