Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2007 Oct 8;27(24):8502–8509. doi: 10.1128/MCB.01382-07

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2007, American Society for Microbiology

PMC Copyright notice

FIG. 4. — Lysine 3016 of ATM is required for ATM to regulate radiosensitivity and dimer-monomer transition. (A) GM5849 A-T cells stably transfected with vector (•), ATM (○), ATM^K3016R (▵), or ATM^K3018R (▪) were irradiated, and cell survival was measured using a clonogenic cell survival assay. Results are means ± SD (n = 3). (B) A-T cells were stably cotransfected with HA- and myc-tagged ATM constructs, such that both HA-ATM and myc-ATM were expressed in the same cell. Cell lines coexpressing HA- and myc-tagged ATM, HA- and myc-tagged ATMkd, or HA- and myc-tagged ATM^K3016A were exposed to bleomycin (5 μM for 30 min). Levels of HA-ATM, myc-ATM, pS1981, and acetylation of lysine K3016 (AcK6) were measured. (C) Cells were treated as described above and then immunoprecipitated with HA antibody. Interaction between HA-ATM and myc-ATM was then assessed by Western blot (WB) analysis to detect HA-ATM and coprecipitating myc-ATM.