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Generation of SIRT3-deficient mice. (A) Gene targeting strategy for the SIRT3 locus. Arrows indicate loxP sites, and Neor indicates the neomycin resistance cassette used to select targeted ES cell clones. Restriction sites are as shown. WT, wild type. (B) SIRT3 PCR genotyping. KO, knockout; Het, heterozygous. (C) SIRT3 protein is absent from liver and brain mitochondria of knockout mice. Protein extracts were probed with antibodies to SIRT3 and F1F0-ATPase subunit α (loading control).
