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. 2000 Nov 13;151(4):945–950. doi: 10.1083/jcb.151.4.945

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© 2000 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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Subcellular localization of FtsZ-GFP fusion proteins. Physcomitrella protoplasts were transiently transfected with the corresponding expression plasmid and GFP fluorescence was visualized by CLSM 2 d after transfection. (a) Protoplast transfected with PpFtsZ1-GFP. (b) Protoplast transfected with PpFtsZ2-GFP. (c) Single chloroplast (detail of a). (d–g) Sections of the chloroplast shown in c demonstrating the localization of PpFtsZ1 solely within chloroplasts. (h) Protoplast transfected with pFtsZ1(1–35)-GFP (incomplete transit peptide) showing cytoplasmic distribution of fusion protein. (i) Protoplast transfected with pFtsZ1(1–93)-GFP (complete transit peptide) showing plastidic distribution of fusion protein. a–c, and h and i, represent overlays of all sections of the object. Chlorophyll fluorescence is shown in red; GFP signals are shown in green. Bars, 5 μm.