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. 2000 Nov 13;151(4):863–878. doi: 10.1083/jcb.151.4.863

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© 2000 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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Induction of RTG-dependent target genes by rapamycin. Wild-type (S288c), rtg1Δ (PLY037), and rtg3Δ (PLY039) cells were grown in MD-glutamine to 0.5 OD₆₀₀/ml and were treated either with drug vehicle (lanes 1, 5, and 9) or with rapamycin for 15 (lanes 2, 6, and 10), 30 (lanes 3, 6, and 9), or 60 (lanes 4, 8, and 12) min. Cells were then harvested and RNA was prepared and analyzed by Northern blotting, probing for the specified mRNAs.