Figure 7.

Regulated nucleocytoplasmic transport of Rtg1 and Rtg3 requires Rtg1, Rtg2, and Rtg3. (A) Rtg1-GFP and Rtg3-GFP were visualized in rtg2Δ (EY0734) cells in the absence (left) or presence (right) of rapamycin. In contrast to wild-type cells, neither Rtg1-GFP nor Rtg3-GFP relocate to the nucleus after rapamycin treatment (compare with Fig. 5 A). (B) Rtg1-GFP was visualized in rtg3Δ (EY0735) cells (top) and Rtg3-GFP was visualized in rtg1Δ (EY0733) cells (bottom). Note that Rtg1-GFP cannot accumulate in the nucleus upon rapamycin treatment in rtg3Δ cells. In contrast, Rtg3-GFP is localized constitutively to the nucleus in rtg1Δ cells, even in the absence of rapamycin addition.