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. 2000 Nov 13;151(4):763–778. doi: 10.1083/jcb.151.4.763

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© 2000 The Rockefeller University Press

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graphic file with name JCB0006047.f10ac.jpg — PKCα activation is sufficient to initiate cell cycle withdrawal in IEC-18 cells. (a) IEC-18 cells were pulsed with PMA for 15 min to selectively downregulate PKCδ and -ε, and PKC isozyme expression was determined by Western blot analysis. (b) PKCδ- and -ε–depleted cells were retreated with PMA for 2 h and analyzed by Western blotting for expression and migration/phosphorylation state of cell cycle regulatory molecules. U, untreated; P, phorbol ester–pulsed cells expressing PKCα but not -δ or -ε; R, PKCα-expressing cells retreated with PMA. Data are representative of three independent experiments. (c) Model of PKCα-mediated intestinal epithelial cell cycle withdrawal (see text for details). (d) Time line of events involved in PKC-mediated cell cycle exit in IEC-18 cells.

graphic file with name JCB0006047.f10d.jpg — PKCα activation is sufficient to initiate cell cycle withdrawal in IEC-18 cells. (a) IEC-18 cells were pulsed with PMA for 15 min to selectively downregulate PKCδ and -ε, and PKC isozyme expression was determined by Western blot analysis. (b) PKCδ- and -ε–depleted cells were retreated with PMA for 2 h and analyzed by Western blotting for expression and migration/phosphorylation state of cell cycle regulatory molecules. U, untreated; P, phorbol ester–pulsed cells expressing PKCα but not -δ or -ε; R, PKCα-expressing cells retreated with PMA. Data are representative of three independent experiments. (c) Model of PKCα-mediated intestinal epithelial cell cycle withdrawal (see text for details). (d) Time line of events involved in PKC-mediated cell cycle exit in IEC-18 cells.