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. 2000 Nov 13;151(4):763–778. doi: 10.1083/jcb.151.4.763

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© 2000 The Rockefeller University Press

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graphic file with name JCB0006047.f6ad.jpg — Analysis of the developmental regulation of cdk and cyclin expression in the small intestinal epithelium. Immunofluorescence analysis of (a) cdk4, (b) cdk2, (c) cyclin E, and (d) cyclin A expression was performed on rat duodenal tissue. All four molecules are readily detectable in crypt cells (C), and undergo a marked decrease in expression at the crypt–villus junction (J), well beyond the point of growth arrest (arrow). Note the variable expression of cyclin A in proliferating cells (d, inset). P, proliferation zone; D, differentiation zone; V, villus. (e) Western blot analysis of cdk and cyclin expression in isolated crypt (C), lower villus (LV), and upper villus (UV) cell populations. Data are representative of at least three independent experiments. Bar, 10 μm.

graphic file with name JCB0006047.f6e.jpg — Analysis of the developmental regulation of cdk and cyclin expression in the small intestinal epithelium. Immunofluorescence analysis of (a) cdk4, (b) cdk2, (c) cyclin E, and (d) cyclin A expression was performed on rat duodenal tissue. All four molecules are readily detectable in crypt cells (C), and undergo a marked decrease in expression at the crypt–villus junction (J), well beyond the point of growth arrest (arrow). Note the variable expression of cyclin A in proliferating cells (d, inset). P, proliferation zone; D, differentiation zone; V, villus. (e) Western blot analysis of cdk and cyclin expression in isolated crypt (C), lower villus (LV), and upper villus (UV) cell populations. Data are representative of at least three independent experiments. Bar, 10 μm.