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. 2000 Nov 13;151(4):739–748. doi: 10.1083/jcb.151.4.739

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© 2000 The Rockefeller University Press

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graphic file with name JCB0006148.f5ad.jpg — Dynein staining at kinetochores after microtubule disassembly. (A and B) Live cell images. A chromosome (arrow) was detached from a metaphase spindle, placed in the cytoplasm, and held there for 10 min. The cell was then lysed in the presence of a calcium-containing microtubule disassembly medium. (C) Superimposed phase–contrast and immunofluorescence images. (D) Immunofluorescence image. (E) Composite showing the kinetochores labeled in C at higher magnification. The kinetochores that had been detached for 10 min (d₁ and d₂) are brighter than the kinetochores that remained attached until calcium treatment (c₁–c₄). Bars, 10 μm.

graphic file with name JCB0006148.f5e.jpg — Dynein staining at kinetochores after microtubule disassembly. (A and B) Live cell images. A chromosome (arrow) was detached from a metaphase spindle, placed in the cytoplasm, and held there for 10 min. The cell was then lysed in the presence of a calcium-containing microtubule disassembly medium. (C) Superimposed phase–contrast and immunofluorescence images. (D) Immunofluorescence image. (E) Composite showing the kinetochores labeled in C at higher magnification. The kinetochores that had been detached for 10 min (d₁ and d₂) are brighter than the kinetochores that remained attached until calcium treatment (c₁–c₄). Bars, 10 μm.