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. 2000 Nov 13;151(4):919–930. doi: 10.1083/jcb.151.4.919

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© 2000 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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Semiquantification of the amounts and ratios of AQP2-S256A and AQP2-S256D-F in coinjections. Copy RNAs encoding AQP2-S256D-F and AQP2-S256A were mixed in the ratios 1:3, 1:2, 1:1, 2:1, and 3:1 (from left to right) and a total of 0. 4 ng was injected into oocytes. 2 d later, the Pf values were determined (see Table ) and total membranes were isolated and immunoblotted for AQP2. The individual signals of AQP2-S256A (SA) and AQP2-S256D-F (SD) were scanned and the amounts of expressed protein were semiquantified (in arbitrary units) using the scanned signals of a twofold dilution series of wt-AQP2 as a standard. From these data, the SD/SA ratio and total AQP2 amounts were calculated.