Table 2.

Expected and Observed Pf Values of Oocytes Coexpressing AQP2-S256D-F and AQP2-256A at Variable Ratios

	Expected Pf
Ratio SD/SA	AAAA	AAAD	AADD	ADDD	DDDD	Observed Pf
0.48	98	75	32	NS	NS	NS
0.51	102	80	36	NS	NS	NS
0.81	91	81	51	14	NS	23 ± 6
1.46	76	74	60	30	NS	25 ± 4
2.39	103	102	94	65	18	62 ± 9
					Control:	8 ± 4

From the data in Fig. 5 and Fig. 6, and Table , the expected Pfs (shown in μm/s) were calculated for when zero, one, two, three, or four AQP2-S256D-F monomers per tetramer would be necessary for a plasma membrane localization of AQP2 (AAAA, AAAD, AADD, ADDD, and DDDD, respectively). The observed Pfs ± SEM (shown in μm/s) for the different coinjections and noninjected oocytes (Control) are mean values of at least 12 oocytes. NS, expected and observed Pf values that were not significantly different from noninjected control oocytes. SD, AQP2-S256D-F; SA, AQP2-256A.