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. 2001 Apr 16;153(2):351–366. doi: 10.1083/jcb.153.2.351

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© 2001 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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Cortical dynamin-rich sites in NIH3T3 cells, which become Abp1 immunopositive after receptor activation, are sites of endocytosis. Dynamin (a) and the endocytic coat component Hip1R (c) colocalize (b, merge) in cells stimulated with growth factors, as observed by confocal microscopy. Colocalization of dynamin (d) and eps15 (f), as seen in the merged image (e). Colocalization of AP2 (g) and Abp1 (i) at puncta at the cell cortex, but not at the leading edge of the cell; as seen in the merged image (h). (j–l) In nonperforated activated cells, the cytosolic pool of Abp1 largely obscures the weak Abp1 immunostaining (l) at sites of endocytosis [here marked by anti–AP2 immunostaining (j)]. Inserts represent enlargements of the areas boxed in a–l. Bars, 10 μm.