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. 2001 Apr 16;153(2):251–262. doi: 10.1083/jcb.153.2.251

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© 2001 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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Kap114 binds to histones H2A and H2B in the cytoplasm. (a) Kap114-PrA and associated proteins were isolated from cytosol by IgG-Sepharose, eluted with a MgCl₂ gradient, separated by SDS-PAGE, and visualized by Coomassie blue staining. The bands representing Kap114-PrA, TBP and histones H2A and H2B are indicated. (b) Cells expressing either H2A-PrA or H2B-PrA were fixed and the PrA moiety detected by indirect immunofluorescence. The coincident DAPI staining is shown. (c) H2A-PrA and associated proteins were isolated from cytosol. Bands representing Kap114p, Nap1p, H2A-PrA, and H2B are indicated. (d) H2B-PrA and associated proteins were isolated as described. Bands representing Kap114p, Nap1p, and H2B-PrA are indicated. Positions of molecular mass standards in kilodaltons are shown.