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. 2001 Apr 16;153(2):251–262. doi: 10.1083/jcb.153.2.251

Figure 4.

Figure 4

H2A and H2B interact with additional Kaps in the absence of Kap114p. (a) H2A-PrA was expressed in a Δkap114 strain. H2A-PrA and associated proteins were isolated as described. Bands representing potential Kaps (*), Nap1p, H2A-PrA, and H2B are indicated. Identical fractions were Western blotted with an antibody to Kap121p, shown below. (b) H2B-PrA was expressed in a Δkap114 strain. H2B-PrA and associated proteins were isolated as described. Bands representing potential Kaps (*), Nap1p, and H2B-PrA are indicated. The fractions were Western blotted with antibodies to Kap121p and Kap123p as indicated below. (U) Unbound supernatant fraction. Positions of molecular mass standards in kilodaltons are shown.