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. 2001 Apr 16;153(2):243–250. doi: 10.1083/jcb.153.2.243

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© 2001 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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Analysis of mouse Dsg 3 expression in transgenic and normal mouse skin. (A) Immunoblot with anti–FLAG antibody indicates expression of mouse Dsg 3-FLAG (arrowhead, ∼160 kD) in extract of transgenic (+) mouse skin. Nontransgenic littermate (−) skin serves as a control. (B) Immunofluorescence of transgenic mouse tail skin for FLAG epitope shows expression of transgene in suprabasilar area of epidermis. (C) Immunofluorescence of nontransgenic mouse tail for mouse Dsg 3 shows expression in the basal and immediate suprabasal layer of epidermis. (D) Immunofluorescence of transgenic mouse tail for mouse Dsg 3 shows expression throughout the epidermis. (B–D) Original magnification 100×. (E) Immunoblotting for mouse Dsg 3 and mouse Dsg 1 from extracts of mouse skin and tongue show that transgenic (scaly) epidermis from founder 1 has a Dsg 3/Dsg 1 ratio similar to normal tongue mucous membrane, whereas transgenic mucous membrane from the same founder expresses excess Dsg 3.