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. 2001 Apr 16;153(2):435–442. doi: 10.1083/jcb.153.2.435

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© 2001 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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The control G1 cells released at 34°C. (A) Photomicrographs taken 105 min after release. Microtubules were detected by indirect immunofluorescence and are shown in red. DNA was visualized by DAPI and shown in blue. (B) FACS^® profile shows that for these cells the time between DNA replication and cytokinesis is ∼75 min. (C) Sister chromatids separate 30 min after budding. 22% of the cells have long spindles in anaphase. Bar, 5 μm.