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. 2001 Apr 16;153(2):435–442. doi: 10.1083/jcb.153.2.435

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© 2001 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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stu2-10/mad2Δ cells do not elongate spindles but otherwise progress normally through the cell cycle at 34°C. stu2-10/mad2Δ cells were grown at 25°C, and then elutriated and released at 34°C. (A) Microphotographs taken 135 min after the release. Microtubules were detected by indirect immunofluorescence and are shown in red. DNA was visualized by DAPI and is shown in blue. (B) FACS^® profile shows that cytokinesis happens with the wild-type timing. (C) Sister chromatids separate 90 min after budding. Less than 1% of stu2-10/mad2Δ cells elongate their spindles. Bar, 5 μm.