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. 1999 Sep 6;146(5):1019–1032. doi: 10.1083/jcb.146.5.1019

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© 1999 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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graphic file with name JCB9903099.f1a.jpg — F-actin is not required for maintenance of spindle orientation. Wild-type cells (DLY5) were grown in YEPD at 30°C, synchronized by addition of 200 mM hydroxyurea for 3 h, harvested, and resuspended in fresh YEPD supplemented with 0 or 100 μM Lat-A. At various times after release from hydroxyurea arrest, the cells were fixed and processed to visualize F-actin, tubulin, and DNA. A, Tubulin (left) and rhodamine-phalloidin (right) staining of cells 30 min after release from hydroxyurea arrest. Bar, 10 μm. B, Kinetics of anaphase. Percent of nuclear division was scored in DAPI-stained cells. C, Spindle orientation was scored as described in Materials and Methods, and reflects counts on both preanaphase and postanaphase spindles. n = 200 for each sample.

graphic file with name JCB9903099.f1bc.jpg — F-actin is not required for maintenance of spindle orientation. Wild-type cells (DLY5) were grown in YEPD at 30°C, synchronized by addition of 200 mM hydroxyurea for 3 h, harvested, and resuspended in fresh YEPD supplemented with 0 or 100 μM Lat-A. At various times after release from hydroxyurea arrest, the cells were fixed and processed to visualize F-actin, tubulin, and DNA. A, Tubulin (left) and rhodamine-phalloidin (right) staining of cells 30 min after release from hydroxyurea arrest. Bar, 10 μm. B, Kinetics of anaphase. Percent of nuclear division was scored in DAPI-stained cells. C, Spindle orientation was scored as described in Materials and Methods, and reflects counts on both preanaphase and postanaphase spindles. n = 200 for each sample.