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. 1999 Sep 6;146(5):955–966. doi: 10.1083/jcb.146.5.955

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© 1999 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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Sympathetic neuron survival in response to NGF and KCl is similar at different cell densities. Sympathetic neuron survival at different densities, as monitored by MTT assays. Neonatal sympathetic neurons were cultured in 50 ng/ml NGF for five days at 350 or 1,500 cells/well, washed free of neurotrophin-containing medium, and then incubated for two days in various concentrations of NGF (ng/ml) or KCl (mM), as indicated on the x-axis. Data is derived from one representative experiment performed in triplicate. In these assays, absolute values are normalized so that the value obtained with 10 ng/ml NGF is considered 100% survival. Error bars represent SD. *Indicate the NGF plus KCl values that are significantly different (P < 0.01, t test) from 2.5 (*) or 5 ng/ml NGF (**). Insets are phase-contrast photographs of representative fields of neurons at the different cell densities.