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. 1999 Sep 6;146(5):1033–1044. doi: 10.1083/jcb.146.5.1033

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© 1999 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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graphic file with name JCB9904118.f3ac.jpg — Elimination of targeting leads to contact growth. (A–C) Sequences of a cell coinjected with Rh-tubulin and Rh-vinculin and pretreated with 20 μM taxol for 30 min (A) to stabilize microtubules, before injection of L61 Rac at time 0:00. Arrowheads indicate peripheral contact sites that grew in the protruding cell edge induced by Rac. Hollow arrowhead indicates a contact that remained in association with a microtubule end and that did not grow. (D–F) control cell injected with Rac but not treated with taxol. Contact sites are targeted and do not enlarge. Bars, 10 μm. Videos available at http://www.jcb. org/cgi/content/full/146/5/1033/ F3/DC1

graphic file with name JCB9904118.f3df.jpg — Elimination of targeting leads to contact growth. (A–C) Sequences of a cell coinjected with Rh-tubulin and Rh-vinculin and pretreated with 20 μM taxol for 30 min (A) to stabilize microtubules, before injection of L61 Rac at time 0:00. Arrowheads indicate peripheral contact sites that grew in the protruding cell edge induced by Rac. Hollow arrowhead indicates a contact that remained in association with a microtubule end and that did not grow. (D–F) control cell injected with Rac but not treated with taxol. Contact sites are targeted and do not enlarge. Bars, 10 μm. Videos available at http://www.jcb. org/cgi/content/full/146/5/1033/ F3/DC1