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Locally applied ML-7 destabilizes microtubules and induces the dissociation of peripheral contact sites. Figure shows video sequence of a cell cotransfected with zyxin-EGFP and tubulin-EGFP that was treated topically, from time 0′00″ with a local application of 2 mM ML-7 through a microneedle (visible in the phase contrast image at 3′12″), over the region indicated by the ellipse. Bar, 10 μM. Video available at http://www.jcb.org/cgi/content/full/146/5/1033/F8/DC1
