Figure 4.
Semiquantitative representation in frequency histograms of intensity score after in situ RT-PCR for CD38 mRNA in osteoclasts incubated either without primer (i) or with CD38 primers without IL-6 treatment (ii) or with IL-6 treatment (10 ng/l, iii, or 10 μg/l, iv). Staining intensity was graded as described in Materials and Methods by an independent blinded observer who scored the intensity from zero (no staining) to 4 (intense staining) in three experiments. The data were analyzed statistically for skewness and shifts were considered significant if P < 0.05.