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. 1999 Sep 6;146(5):1161–1172. doi: 10.1083/jcb.146.5.1161

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© 1999 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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Western blotting after SDS-PAGE of osteoclast plasma membranes (OC-PM) (30 μg protein) and osteoblast postnuclear membranes (OB-PNM) (50 μg protein) using an antagonist anti-CD38 antibody (Sigma Chemical Co.). We identified 46-kD bands with an additional lower molecular weight band in OC-PMs. The osteoclast preparations were >99% pure as assessed by TRAP staining (see Materials and Methods).