Figure 2.

Targeted disruption of the afadin gene. (A) Restriction maps of the wild-type allele, the targeting vector, and the targeted allele of the afadin gene. Filled boxes, exons. S, SacI; H, HindIII; Sp, SphI; X, XbaI; P1, PCR primer, 5′-TTCTAGGATTTGGAGTTTCAT-3′; and P2, PCR primer, 5′-GGTCAGGACACAGTCTTCACT-3′. (B) Southern blot analysis of ES clones. HindIII-digested DNAs derived from ES cells were hybridized with the 5′ or 3′ probe. (C) Southern blot analysis of mice. The afadin^+/− mice were intercrossed. HindIII-digested DNAs derived from tails of the progeny were hybridized with the the 3′ probe. +/+, wild type; and +/−, heterozygous mutant.