Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 1999 Sep 6;146(5):941–954. doi: 10.1083/jcb.146.5.941

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 1999 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

PMC Copyright notice

hBUBR1 expression, phosphorylation, and kinase activity during the cell cycle. (A) K562 and Hela cells were separated according to their position in the cell cycle by centrifugal elutriation. hBUBR1 was immunoprecipitated from equal amounts of protein from each fraction, and then tested for autokinase activity (top) or probed with hBUBR1 antibodies to compare its cell cycle expression profile (middle and bottom). (B) Comparison of autokinase activity (top) and levels of hBUBR1 (middle) and hBUB3 found in hBUBR1 immunoprecipitates from asynchronous interphase cells (A) or cells blocked in mitosis by nocodazole (N). (C) hBUBR1 is hyperphosphorylated in mitosis. hBUBR1 immunoprecipitated from mitotically blocked Hela cells were incubated in the presence (lane 1) or absence (lane 2) of lambda protein phosphatase (λPP). (lanes 3–8) Comparison of hyperphosphorylation status of hBUBR1 in normal mitotic Hela cells (lane 3, M), nocodazole-blocked mitotic cells (lane 4, N), or cells released from the nocodazole block for 1, 2, 3, and 4 h (lanes 5–8, respectively). Cells were determined to have reentered G1 by phase-contrast microscopy.