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. 1999 Sep 6;146(5):917–928. doi: 10.1083/jcb.146.5.917

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© 1999 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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In purified vaults, p193 is a PARP that ADP-ribosylates itself and the MVP. Vaults (3 μg) purified from rat liver were incubated in the presence of [³²P]NAD+ (as indicated) and the products separated by SDS-PAGE followed by Coomassie blue staining (left panel), and by autoradiography (right panel). Reactions were supplemented with either unlabeled NAD+ (Cold Chase) or with the PARP inhibitor 3ABA (Inhibitor). Arrows indicate the specific ADP-ribosylated proteins. The bracket indicates the smear of higher molecular weight ADP-ribosylated products.